Case 7: Additional Pathology

Case 7 Index

Finally, some additional preparations are ready! Take a look below…

EM (Electron Microscopy)

Which of the following do you see above (more than one may be correct)?
Tubuloreticular inclusion (TRI)
Well  done! TRIs are seen within the endothelial cell and are thought to arise from the endoplasmic reticulum.
Subepithelial deposits
We don’t see any significant subepithelial deposits here, try again!
Subendothelial deposits
Correct! Here we see deposits along the basement membrane on the side of the capillary lumen, therefore in the subendothelial space!
Duplication or “tram-tracking” of the basement membrane
The basement membrane appears to be intact here, with only one layer visible. Try again!
Mesangial deposits
Though there are mesangial changes and there may be mesangial deposits, the mesangium is not visualized above.

IF (Immunofluorescence)


Glomerular and TBM IgG Deposits


Glomerular C1q Deposition


Granular, Glomerular IgG Deposits


Glomerular C1q Deposition

What do you see above on immunofluorescence (IF) for this patient?
Granular basement membrane staining
Correct! This is consistent with immune deposition disease and our many subendothelial deposits that we saw on EM. We also see staining of the mesangial space.
Linear basement membrane staining
We do not see a linear pattern here, which would be inconsistent with the subendothelial deposits and unaffected basement membrane as seen on EM in this patient. Try again!
Negative IF staining
Negative IF would appear as a dark shadow. In addition, we see subendothelial deposits on EM above, implying the presence of immune deposits. Negative IF would suggest the absence of immune deposits.

Click here if you’re confident in your final diagnosis!

Case 7 Index
Case 7 Introduction
Case 7 Physical Exam
Case 7 Diagnostic Testing
Case 7 Pathology